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k562 nuclear cell extracts  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology k562 nuclear cell extracts
    K562 Nuclear Cell Extracts, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k562 nuclear cell extracts/product/Santa Cruz Biotechnology
    Average 93 stars, based on 24 article reviews
    k562 nuclear cell extracts - by Bioz Stars, 2026-03
    93/100 stars

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    Electromobility shift assays (EMSAs) suggest that rs61746197 A > G is a functional SNP. ( A ) Ten micrograms of unstimulated nuclear extract from <t>K562</t> cells was incubated with reference (Ref, R) or alternative (Alt, A) probe as indicated. Cold Ref probe titrates signal away from Alt at a lower stoichiometric ratio than the converse (arrow and compare lanes 11 – 13 with 6 – 8 ). ( B ) Unstimulated extract ( left panel, U) exhibits a markedly stronger baseline shift than TPA stimulated extract ( right panel, S). Unstimulated nuclear extract (U) when incubated with labeled alternative probe (A) produces a novel secondary shift not observed with either the reference probe (R) or the TPA stimulated extract (S). Addition of RelA–p65 antibody abrogates this secondary shift (arrow).
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    Electromobility shift assays (EMSAs) suggest that rs61746197 A > G is a functional SNP. ( A ) Ten micrograms of unstimulated nuclear extract from <t>K562</t> cells was incubated with reference (Ref, R) or alternative (Alt, A) probe as indicated. Cold Ref probe titrates signal away from Alt at a lower stoichiometric ratio than the converse (arrow and compare lanes 11 – 13 with 6 – 8 ). ( B ) Unstimulated extract ( left panel, U) exhibits a markedly stronger baseline shift than TPA stimulated extract ( right panel, S). Unstimulated nuclear extract (U) when incubated with labeled alternative probe (A) produces a novel secondary shift not observed with either the reference probe (R) or the TPA stimulated extract (S). Addition of RelA–p65 antibody abrogates this secondary shift (arrow).
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    Electromobility shift assays (EMSAs) suggest that rs61746197 A > G is a functional SNP. ( A ) Ten micrograms of unstimulated nuclear extract from K562 cells was incubated with reference (Ref, R) or alternative (Alt, A) probe as indicated. Cold Ref probe titrates signal away from Alt at a lower stoichiometric ratio than the converse (arrow and compare lanes 11 – 13 with 6 – 8 ). ( B ) Unstimulated extract ( left panel, U) exhibits a markedly stronger baseline shift than TPA stimulated extract ( right panel, S). Unstimulated nuclear extract (U) when incubated with labeled alternative probe (A) produces a novel secondary shift not observed with either the reference probe (R) or the TPA stimulated extract (S). Addition of RelA–p65 antibody abrogates this secondary shift (arrow).

    Journal: Cold Spring Harbor Molecular Case Studies

    Article Title: 1q21.1 deletion and a rare functional polymorphism in siblings with thrombocytopenia-absent radius–like phenotypes

    doi: 10.1101/mcs.a004564

    Figure Lengend Snippet: Electromobility shift assays (EMSAs) suggest that rs61746197 A > G is a functional SNP. ( A ) Ten micrograms of unstimulated nuclear extract from K562 cells was incubated with reference (Ref, R) or alternative (Alt, A) probe as indicated. Cold Ref probe titrates signal away from Alt at a lower stoichiometric ratio than the converse (arrow and compare lanes 11 – 13 with 6 – 8 ). ( B ) Unstimulated extract ( left panel, U) exhibits a markedly stronger baseline shift than TPA stimulated extract ( right panel, S). Unstimulated nuclear extract (U) when incubated with labeled alternative probe (A) produces a novel secondary shift not observed with either the reference probe (R) or the TPA stimulated extract (S). Addition of RelA–p65 antibody abrogates this secondary shift (arrow).

    Article Snippet: EMSAs were performed using TPA-stimulated and unstimulated nuclear extracts of K562 cells (Active Motif).

    Techniques: Functional Assay, Incubation, Labeling